KAJIAN LITERATUR SPESIFIKASI MUTU FISIKOKIMIA DAN MIKROBIOLOGIS WATER KEFIR DENGAN VARIASI KONSENTRASI SUBSTRAT DAN STARTER

Veronica P. Effendi, Adolf J.N. Parhusip

Abstrak


ABSTRAK: Water kefir adalah salah satu produk minuman dengan bahan dasar larutan gula atau sari nabati yang melalui proses fermentasi  dengan bibit water kefir dengan ciri khas rasa asam, sedikit rasa manis, serta mengandung alkohol dan karbon dioksida. Salah satu mikroorganisme yang terkandung dalam bibit water kefir, yaitu bakteri asam laktat memiliki peran fungsional sebagai probiotik yang bermanfaat bagi kesehatan saluran pencernaan dan juga bekerja secara sinergis dengan khamir dalam proses fermentasi water kefir. Selain mengandung probiotik, water kefir juga merupakan diversifikasi produk pangan yang dapat menjadi alternatif bagi kelompok vegan, vegetarian, dan individu yang memiliki alergi terhadap produk olahan susu. Kajian literatur dibuat dengan tujuan untuk mempelajari pengaruh variasi penambahan konsentrasi substrat dan starter dalam pembuatan water kefir terhadap mutu fisikokimia dan mutu mikrobiologis water kefir. Data dalam kajian literatur diperoleh dari jurnal internasional dan nasional terakreditasi dengan minimal ranking Q4 dan Sinta 4. Melalui kajian literatur yang dilakukan dapat disimpulkan bahwa peningkatan konsentrasi starter hingga 15% dapat menurunkan nilai pH dan meningkatkan kadar alkohol, total asam tertitrasi, viskositas, karbon dioksida, total bakteri asam laktat serta total khamir. Peningkatan substrat hingga konsentrasi 9% dapat menurunkan nilai pH dan viskositas serta meningkatkan kadar alkohol, total asam tertitrasi, karbon dioksida, total bakteri asam laktat serta total khamir. Plasmolisis sel-sel mikroorganisme bibit water kefir dapat terjadi pada penambahan substrat dengan konsentrasi 10% atau lebih sehingga pH meningkat dan kadar alkohol, karbon dioksida, total khamir, dan total bakteri asam laktat pada produk akhir yang dihasilkan menurun.


ABSTRACT: Water kefir is a beverage made from sugar or plant based solution that is fermented with water kefir seeds that has sour and slightly sweet taste characteristics, contains alcohol and carbon dioxide. The microorganisms contained in water kefir seeds such as lactic acid bacteria have a functional role as beneficial probiotics that can promote digestive tract health and work synergistically with yeast in water kefir fermentation. Water kefir is also a form of diversity of food product that can be an alternative for vegans, vegetarians, and individuals who have certain allergies to dairy products. This literature review was made to study the effect of variations of substrate and starter concentrations used in water kefir fermentation on the physicochemical quality and microbiological of water kefir. The data in this literature review were obtained from international and national journals with a minimum ranking of Q4 and Sinta 4. In conclusion, increasing the starter concentration up to 15% can reduce the pH value and increase the alcohol content, total titrated acid, viscosity, carbon dioxide, total lactic acid bacteria and total yeast. The addition of substrate up to 9% can reduce the pH value and viscosity and increase the alcohol content, total titrated acid, carbon dioxide, total lactic acid bacteria and total yeast. Plasmolysis in microorganisms cells happened with the addition of a substrate with a concentration of 10% or more causing the pH value to increase and decreasing the levels of alcohol, carbon dioxide, total yeast, and total lactic acid bacteria in the final product.

Kata Kunci


water kefir, sinergisme, konsentrasi substrat, konsentrasi starter

Teks Lengkap:

PDF

Referensi


Aristya, A.L., Legowo, A.M., Al-Baari, A.N. 2013. Karakteristik fisik, kimia, dan mikrobiologis kefir susu kambing dengan penambahan jenis dan konsentrasi gula yang berbeda. Jurnal Aplikasi Teknologi Pangan, 2(3), Hal. 139-143.

Ayivi, R.D., Gyawali, R., Krastanov, A., Aljaloud, S.O., Worku, M., Tahergorabi, R., Da Silva, R.C., Ibrahim, S.A. 2020. Lactic acid bacteria: food safety and human health application. Dairy, 1(3), pp. 202-232. DOI: https://doi.org/10.3390/dairy1030015.

Azizah, R., Restuhadi. F., dan Rossi, E. 2014. Kajian penggunaan tween80™ pada pelbagai konsentrasi nira nipah kental dalam proses fermentasi bioetanol. Journal Online Faculty of Agriculture Students, 1(1), Hal. 1-8.

Bryan, A., Hart, C., Howell, A., Wise, M., dan Roberts, B. 2018. Glucose concentrations effect on rate of fermentation in yeast. Journal of Undergraduate Biology Laboratory Investigation, pp. 1-4.

BSN. 2009. Minuman susu fermentasi berperisa. Badan Standarisasi Nasional, SNI 7552:2009.

Burns, D.T., Johnston, E.L., dan Walker, M.J. 2020. Authenticity and the potability of coconut water - a critical review. Journal of AOAC International, 103(3), pp. 800-806. DOI: 10.1093/jaocint/qsz008.

CAC. 2003. CODEX standards for fermented milks. Codex Alimentarius Comission, CODEX STAN 243-2003.

Da Costa, S.L., Rossi, N.P., dan Maldonado, R.R. 2013. Evaluation of lactose in milk and dairy products. International Journal for Innovation Education and Research, 1(3), pp. 1-4.

Dahunsi, A.T.A., Dahunsi, S.O., dan Olayanju, A. 2019. Synergistic microbial interactions between lactic acid bacteria and yeasts during production of Nigerian indigenous fermented foods and beverages. Food Control, 110, pp. 1- 30.

Danilovic, B., Dordevic, N., dan Savic, D. 2019. Microbiological and chemical changes during two-phase fermentation of kefir. Advanced Technologies, 8(1), pp. 5-9.

Dertli, E., dan Con, A.H. 2017. Microbial diversity of traditional kefir grains and their role on kefir aroma. LWT Food Science and Technology, 85, pp. 151-157.

Dewi, A.C., Rahardjo, A.H.D., Setyawardani, T., dan Subagja, H. 2019. Study on kefir grain concentration and the different length of storage on the physicochemical of goat milk kefir. Journal of Physics: Conference Science, 1569, pp.1-5. DOI: 10.1088/1742-6596/1569/3/032001

FSANZ. 2015. Fermented milk products. Food Standards Australia New Zealand, ANZFS Code Standard 2.5.3.

Guzel-Seydim, Z.B., Gokirmakli, C., dan Greene, A.K. 2021. A comparison of milk kefir and water kefir: physical, chemical, microbiological and functional properties. Trends in Food Science and Technology, 113, pp. 42-53.

Insani, H., Rizqiati, H., dan Prataman, Y. 2018. Pengaruh variasi konsentrasi sukrosa terhadap total khamir, total padatan terlarut, kadar alkohol dan mutu hedonik pada water kefir buah naga merah (Hyloreceus polyrhizus). Jurnal Teknologi Pangan, 2(2), Hal. 90–95.

Ismail, Y. S., Yulvizar, C. & Mazhitov, B. 2018. Characterization of lactic acid bacteria from local cow’s milk kefir. IOP Conference Series: Earth and Environmental Science, 130, Hal. 1-8.

JETRO.2011. Specifications and standards for foods, food additives, etc. under the food sanitation act. Japan External Trade Organization.

KEBS. 2018. Fermented (cultured) milks – specification. Kenya Beurau of Standards, Kenya Standard DKS 941:2018.

Kopecka, J., Matoulkova., dan Nemec, M. 2012. Yeast And Its Uses. Kvasny Prum, 58, pp.326-334. DOI:10.18832/kp2012029.

Korukluoglu, M., Arik, G., Erdogan, C., dan Kocakoglu, S. 2017. Screening of antagonistic/synergistic effect between lactic acid bacteria (lab) and yeast strains isolated from kefir. International Journal of Nutrition and Food Engineering, 11(1), pp. 282-288.

Kurniati, T., Windayani, N., dan Listiawati, M. 2020. Total lactic acid, protein, fat, and carbohydrates in curd kefir and cow colostrum kefir. Jurnal Biodjati, 5(2), Hal. 271-280.

Laureys, D., Aerts, M., Vandamme, P., Vuyst L.D. 2018. Oxygen and diverse nutrients influence the water kefir fermentation process, Food Microbiology, 75, pp. 351-361. DOI: 10.1016/ j.fm.2018.02.007.

Laureys, D., dan Vuyst, LD. 2016. The water kefir grain inoculum determines the characteristics of the resulting water kefir fermentation process. Journal of Applied Microbiology, 122, pp. 719-732. DOI :10.1111/jam.1337.

Lynch, K.M., Wilkinson, S., Daenen, L., dan Arendt, E.K. 2021. An update on water kefir: Microbiology, composition and production. International Journal of Food Microbiology, 345(109128), pp. 1- 18. DOI: 10.1016/j.ijfoodmicro.2021.109128.

Magra, T.I., Antoniou, K.D., dan Psomas, E.I. 2012. Effect of milk fat, kefir grain inoculum and storage time on the flow properties and microbiological characteristics of kefir. Journal of Texture Studies, 43(4), pp. 1-10. DOI: 10.1111/j.1745-4603.2011.00343.x.

Margareth, L.L., Nurwantoro, dan Rizqiati, H. 2020. Effect of different kefir grain starter concentration on yield, ph, co2 content, and organoleptic properties of buffalo milk kefir. Journal of applied food technology, 7(1), Hal. 15-18.

Narayan, A., Bhattacharjee, K., dan Naganathan, A.N. 2019. Thermally versus chemically denatured protein states. Biochemistry, 58, pp. 2519-2523.

Nur’utami, D.A., Pertiwi, S.R.R., dan Syarifah, N. 2018. formulasi kefir rendah lemak dari kacang bambara (Vigna subterranae) dengan variasi waktu fermentasi dan konsentrasi starter. Jurnal Agroindustri Halal, 4 (2), Hal. 192 – 199.

Pourahmad, R., Moghimi, A., Dadkhah, S. dan Assadi, M.M. 2011. Evaluation of flavor and aroma compounds amounts in kefir from soymilk. World Applied Science Journal, 15(5), pp. 673-676.

Prastiwi, V.F., Bintoro, V.P., dan Rizqiati, H. 2018. Sifat mikrobiologi, nilai viskositas dan organoleptik kefir optima dengan penambahan high fructose syrup (HFS). Jurnal Teknologi Pangan, 2(1), Hal. 27-32.

Prastujati, A.U., Hilmi, M., dan Khirzin, M.H. 2018. Pengaruh konsentrasi starter terhadap kadar alkohol, pH, dan total asam tertitrasi (TAT) whey kefir. Jurnal Imu Peternakan Terapan, 1(2), Hal. 63-69.

Purbasari, A., Pramono, Y.B., dan Abduh, S.B.M. 2014. Nilai ph, kekentalan, citarasa asam, dan kesukaan pada susu fermentasi dengan perisa alami jambu air (Syzygium sp). Jurnal Aplikasi Teknologi Pangan, 3(4), pp. 174-177.

Randazzo, W., Corona, O., Guarcello, R., Francesca, N., Germana, M.A., Erten, H., Moschetti, G., dan Settani, L. 2016. Development of new non-dairy beverages from Mediterranean fruit juices fermented with water kefir microorganisms. Food Microbiology, 54, pp. 40-51.

Rizqiati, H., Mulyani, S., dan Ramadhanti, D.L. 2021. Pengaruh variasi konsentrasi sukrosa terhadap total bakteri asam laktat, ph, kadar alkohol dan hedonik water kefir belimbing manis (Averrhoa carambola). Jurnal Ilmiah Sains, 21(1), Hal. 54-62. DOI: https://doi.org/10.35799/jis.21.1.2021.31160.

Rosiana, E., Nurliana, dan Armansyah, T. 2013. Kadar asam laktat dan derajat asam kefir susu kambing yang di fermentasi dengan penambahan gula dan lama inkubasi yang berbeda. Jurnal Medika Veterinarian, 7(2), pp. 87-90.

Safitri, M.F., dan Swarastuti, A. 2013. Kualitas kefir berdasarkan konsentrasi kefir grain. Jurnal Aplikasi Teknologi Pangan, 2(2), Hal. 87-92.

Stadie, J., Gilitz, A., Ehrmann, M.A., dan Vogel, R.F. 2013. Metabolic activity and symbiotic interactions of lactic acid bacteria and yeasts isolated from water kefir. Food Microbiology, 35(2), pp. 92-98. DOI: 10.1016/j.fm.2013.03.009.

Suharman, Sutakwa, A., dan Nadia, L.S. Effects of sucrose addition to lactic acid concentrations and lactic acid bacteria population of butterfly pea (Clitoria Ternatea L.) yogurt. Journal of Physics : Conference Science, 1823, pp. 1-5. DOI: 10.1088/1742-6596/1823/1/012038.

Sulmiyati, Said, N.S., Fahrodis, D.U., Malaka, R., dan Maruddin, F. 2019. The physicochemical, microbiology, and sensory characteristics of kefir goat milk with different levels of kefir grain. Tropical Animal Science Journal, 42(2), Hal. 152-158. DOI: https://doi.org/10.5398/tasj.2019.42.2.152.

Supriyono, T., Murwani, R., dan Nurrahman. 2014. Kandungan beta karoten,polifenol total dan aktifitas “merantas” radikal bebas kefir susu kacang hijau (Vigna radiata) oleh pengaruh jumlah starter(Lactobacillus bulgaricus dan Candida kefir) dan konsentrasi glukosa. Jurnal Gizi Indonesia, 2(2), pp. 65-71.

Surja, L.L., Dwiloka, B., dan Rizqiati, H. 2019. Effect of high fructose syrup (HFS) addition on chemical and organoleptic properties of green coconut water kefir. Journal of applied food technology, 6(1), pp. 3-8.

Torres, A.M., Abrocio, S.G., Del Orbe, P.H., Tanaca, L.V., dan Rodriguez. C.H. 2016. Inferring the role of microorganisms in water kefir fermentations. International Journal of Food Science and Technology, pp. 2-12.

Triwibowo, B., Wicaksono, R., Antika, Y., Ermi, S., Jarmiati, A., Setiadi, A.A., Syahriar, R. 2019. The effect of kefir grain concentration and fermentation duration on characteristics of cow milk-based kefir. Journal of Physics: Conference Series, 1444(012001), Hal. 1-6. DOI: 10.1088/1742- 6596/1444/1/012001

Wahono, S.K., Damayanti, E., Rosyida, V.T., Sadyastuti, E.I. 2011. Laju pertumbuhan Saccharomyces cerevisiae pada proses fermentasi pembentukan bioetanol dari biji sorgum (Sorghum bicolor L.). Seminar Rekayasa Kimia Dan Proses, Hal. 3-6.

Walker, G.M., dan Stewart, G.G. 2016. Saccharomyces cerevisiae in the production of fermented beverages. Beverages, 2(30), pp. 1-12. DOI: 10.3390/beverages2040030.

Yang, E., Fan, L., Yan, J., Jiang, Y., Doucette, C., Fillmore, S., dan Walker, B. 2018. Influence of culture media, pH and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria. AMB Express, 8(10), pp. 1-14. DOI: 10.1186/s13568-018-0536-0.




DOI:

https://doi.org/10.36441/jtepakes.v3i2.558

Article Metrics

Abstrak views : 1952 times
PDF views : 1520 times

Dimension Citation Metrics

Refbacks

  • Saat ini tidak ada refbacks.


Jurnal Ini Terindeks di:

width= width= width= width= 

width=

 


Lisensi Creative Commons
Ciptaan disebarluaskan di bawah Lisensi Creative Commons Atribusi 4.0 Internasional.

 

 

 

View My Stats